Journal:

Article Title: Fusobacterium nucleatum Increases Collagenase 3 Production and Migration of Epithelial Cells

doi: 10.1128/IAI.73.2.1171-1179.2005

Figure Lengend Snippet: F. nucleatum preferentially binds to marginal cells of artificial epithelial wounds and increases their migration. Scratch wounds were created to confluent HaCaT cell cultures, and F. nucleatum cells were added to the culture medium (260 bacteria per cell) for different time periods. Epifluorescence of cultures detected with anti-F. nucleatum antibody after 6 h (A), 24 h (B) and 48 h (C). Light microscopic images of wounds at 48 h in the presence of TGF-β indicate faster closure of the epithelial wound sheets in F. nucleatum-treated cultures than in control cultures (D).

Article Snippet: When added to cultures over 90% of the bacteria were dead. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterium Strain Source Porphyromonas gingivalis RHI 3610 (ATCC 33277) Gingival pocket Porphyromonas endodontalis AHN 4610 Tonsils RHI 3609 (ATCC 35406) Root canal Prevotella buccae AHN 10652 Gingival pocket Prevotella oris AHN 19550 Root canal Prevotella intermedia AHN 18240 Odontogenic abscess Prevotella denticola AHN 10695 Gingival pocket RHI 3606 (ATCC 33185) Maxillary atrium Prevotella nigrescens AHN 18826 Odontogenic abscess Prevotella loeshii AHN 10628 Saliva Actinobacillus actinomycetemcomitans JP-2 Gingival pocket Fusobacterium nucleatum AHN 19959 Odontogenic abscess RHI 4185 (ATCC 25586) Cervicofacial lesion Fusobacterium necrophorum AHN 12454 Odontogenic abscess RHI 3624 (ATCC 25286) Bovine liver abscess Mitsuokella dentalis AHN 12573 Root canal Campylobacter rectus AHN 19728 Odontogenic abscess Open in a separate window Bacteria used in this study and their sources

Techniques: Migration, Bacteria, Control

Journal:

Article Title: Fusobacterium nucleatum Increases Collagenase 3 Production and Migration of Epithelial Cells

doi: 10.1128/IAI.73.2.1171-1179.2005

Figure Lengend Snippet: Intracellular F. nucleatum colocalizes with lysosomal membrane structures in cells at the leading edge of the epithelial sheets. Scratched HaCaT cell cultures treated for 48 h with F. nucleatum (ATCC 25586; 260 bacteria per cell) were simultaneously immunostained for F. nucleatum and lysosomal membrane protein LAMP-1, followed by two fluorescent secondary antibodies, Alexa-488 and Alexa-546, respectively. Samples were analyzed by laser confocal microscopy, and optical z-axis sections were recorded. One optical section from the basal, mid-, and apical regions of the cells were presented together. Red channel (A and B) corresponding to Alexa-546 fluorescence reveals F. nucleatum invasion, and green channel (C), corresponding to Alexa-488 fluorescence, reveals LAMP-1 expression. Red and green images were merged into an RGB file (D) showing colocalization (yellow/orange) of the two signals.

Article Snippet: When added to cultures over 90% of the bacteria were dead. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterium Strain Source Porphyromonas gingivalis RHI 3610 (ATCC 33277) Gingival pocket Porphyromonas endodontalis AHN 4610 Tonsils RHI 3609 (ATCC 35406) Root canal Prevotella buccae AHN 10652 Gingival pocket Prevotella oris AHN 19550 Root canal Prevotella intermedia AHN 18240 Odontogenic abscess Prevotella denticola AHN 10695 Gingival pocket RHI 3606 (ATCC 33185) Maxillary atrium Prevotella nigrescens AHN 18826 Odontogenic abscess Prevotella loeshii AHN 10628 Saliva Actinobacillus actinomycetemcomitans JP-2 Gingival pocket Fusobacterium nucleatum AHN 19959 Odontogenic abscess RHI 4185 (ATCC 25586) Cervicofacial lesion Fusobacterium necrophorum AHN 12454 Odontogenic abscess RHI 3624 (ATCC 25286) Bovine liver abscess Mitsuokella dentalis AHN 12573 Root canal Campylobacter rectus AHN 19728 Odontogenic abscess Open in a separate window Bacteria used in this study and their sources

Techniques: Membrane, Bacteria, Confocal Microscopy, Fluorescence, Expressing

Journal:

Article Title: Fusobacterium nucleatum Increases Collagenase 3 Production and Migration of Epithelial Cells

doi: 10.1128/IAI.73.2.1171-1179.2005

Figure Lengend Snippet: Time dependence of collagenase 3 secretion by epithelial cells treated with F. nucleatum. The bacteria were added to serum-free culture medium of semiconfluent HaCaT cells (260 bacteria per cell). Aliquots of the medium were analyzed for collagenase 3 at different time points by Western blotting. Control cells (CTL) were cultured for 24 h in the absence of the bacteria. Relative levels of pro-collagenase 3 protein were quantified by densitometric scanning.

Article Snippet: When added to cultures over 90% of the bacteria were dead. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterium Strain Source Porphyromonas gingivalis RHI 3610 (ATCC 33277) Gingival pocket Porphyromonas endodontalis AHN 4610 Tonsils RHI 3609 (ATCC 35406) Root canal Prevotella buccae AHN 10652 Gingival pocket Prevotella oris AHN 19550 Root canal Prevotella intermedia AHN 18240 Odontogenic abscess Prevotella denticola AHN 10695 Gingival pocket RHI 3606 (ATCC 33185) Maxillary atrium Prevotella nigrescens AHN 18826 Odontogenic abscess Prevotella loeshii AHN 10628 Saliva Actinobacillus actinomycetemcomitans JP-2 Gingival pocket Fusobacterium nucleatum AHN 19959 Odontogenic abscess RHI 4185 (ATCC 25586) Cervicofacial lesion Fusobacterium necrophorum AHN 12454 Odontogenic abscess RHI 3624 (ATCC 25286) Bovine liver abscess Mitsuokella dentalis AHN 12573 Root canal Campylobacter rectus AHN 19728 Odontogenic abscess Open in a separate window Bacteria used in this study and their sources

Techniques: Bacteria, Western Blot, Control, Cell Culture

Journal:

Article Title: Fusobacterium nucleatum Increases Collagenase 3 Production and Migration of Epithelial Cells

doi: 10.1128/IAI.73.2.1171-1179.2005

Figure Lengend Snippet: Up-regulation of epithelial cell-associated collagenase 3 expression by F. nucleatum. HaCaT cells were cultured to full confluence and then wounded by scratching with a pipette tip. They were then cultured in the presence of F. nucleatum (ATCC 25586; 260 bacteria per cell) for 12 h. The cells were washed and simultaneously immunostained for F. nucleatum and collagenase 3, followed by two fluorescent secondary antibodies, Alexa-488 and Alexa-546, respectively. Alexa-546 fluorescence reveals F. nucleatum invasion in certain cells in the wound margin (A, arrows). Alexa-488 fluorescence reveals collagenase 3 staining in the infected cells (B, arrowheads). Combination of the two images shows no clear colocalization of the signals (C).

Article Snippet: When added to cultures over 90% of the bacteria were dead. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterium Strain Source Porphyromonas gingivalis RHI 3610 (ATCC 33277) Gingival pocket Porphyromonas endodontalis AHN 4610 Tonsils RHI 3609 (ATCC 35406) Root canal Prevotella buccae AHN 10652 Gingival pocket Prevotella oris AHN 19550 Root canal Prevotella intermedia AHN 18240 Odontogenic abscess Prevotella denticola AHN 10695 Gingival pocket RHI 3606 (ATCC 33185) Maxillary atrium Prevotella nigrescens AHN 18826 Odontogenic abscess Prevotella loeshii AHN 10628 Saliva Actinobacillus actinomycetemcomitans JP-2 Gingival pocket Fusobacterium nucleatum AHN 19959 Odontogenic abscess RHI 4185 (ATCC 25586) Cervicofacial lesion Fusobacterium necrophorum AHN 12454 Odontogenic abscess RHI 3624 (ATCC 25286) Bovine liver abscess Mitsuokella dentalis AHN 12573 Root canal Campylobacter rectus AHN 19728 Odontogenic abscess Open in a separate window Bacteria used in this study and their sources

Techniques: Expressing, Cell Culture, Transferring, Bacteria, Fluorescence, Staining, Infection

Journal:

Article Title: Fusobacterium nucleatum Increases Collagenase 3 Production and Migration of Epithelial Cells

doi: 10.1128/IAI.73.2.1171-1179.2005

Figure Lengend Snippet: Effect of p38 MAP kinase inhibitor on F. nucleatum-induced collagenase 3 expression in epithelial cells. HaCaT cells were preincubated for 30 min with 10 μM SB203580 (inhibitor of p38) and then cultured in the presence of F. nucleatum (ATCC 25586; 160 bacteria per cell) for 24 h. Culture medium was analyzed by Western blotting, using anti-collagenase 3 antibodies, and RNA extracted from the cells (20 μg) was analyzed for collagenase 3 mRNA levels by Northern blotting.

Article Snippet: When added to cultures over 90% of the bacteria were dead. table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Bacterium Strain Source Porphyromonas gingivalis RHI 3610 (ATCC 33277) Gingival pocket Porphyromonas endodontalis AHN 4610 Tonsils RHI 3609 (ATCC 35406) Root canal Prevotella buccae AHN 10652 Gingival pocket Prevotella oris AHN 19550 Root canal Prevotella intermedia AHN 18240 Odontogenic abscess Prevotella denticola AHN 10695 Gingival pocket RHI 3606 (ATCC 33185) Maxillary atrium Prevotella nigrescens AHN 18826 Odontogenic abscess Prevotella loeshii AHN 10628 Saliva Actinobacillus actinomycetemcomitans JP-2 Gingival pocket Fusobacterium nucleatum AHN 19959 Odontogenic abscess RHI 4185 (ATCC 25586) Cervicofacial lesion Fusobacterium necrophorum AHN 12454 Odontogenic abscess RHI 3624 (ATCC 25286) Bovine liver abscess Mitsuokella dentalis AHN 12573 Root canal Campylobacter rectus AHN 19728 Odontogenic abscess Open in a separate window Bacteria used in this study and their sources

Techniques: Expressing, Cell Culture, Bacteria, Western Blot, Northern Blot

Journal: Scientific Reports

Article Title: Parvimonas micra as a putative non-invasive faecal biomarker for colorectal cancer

doi: 10.1038/s41598-020-72132-1

Figure Lengend Snippet: Increased levels of specific microbial markers are detected in faeces of CRC patients. Scatter plots are used to illustrate the relative levels of ( A ) P. micra ( Pm ), ( B ) F. nucleatum ( Fn ), and ( C ) clbA + bacteria ( clbA ) in faeces of control patients, and patients diagnosed with dysplasia or CRC from the FECSU and U-CAN cohorts. Horizontal lines indicate mean relative expression calculated by the 2 -ΔCq method with the total microbial 16S rRNA gene DNA as reference.

Article Snippet: P. micra 20,468 (DSZM), F. nucleatum subsp. nucleatum Knorr (ATCC 25,586), and Escherichia coli Nissle 1917 were used as positive controls for the respective PCR reactions.

Techniques: Bacteria, Control, Expressing

Journal: Scientific Reports

Article Title: Parvimonas micra as a putative non-invasive faecal biomarker for colorectal cancer

doi: 10.1038/s41598-020-72132-1

Figure Lengend Snippet: ROC curves displaying the specificity and the sensitivity for P. micra ( Pm ), F. nucleatum ( Fn ), and clbA + bacteria ( clbA ) to detect CRC. ROC-curves were calculated using the levels for the specific marker as indicated and cancer/no cancer. The levels of a specific marker in each sample was given as a relative quantification calculated by the 2 -ΔCt method with the total microbial 16S rRNA gene DNA as reference.

Article Snippet: P. micra 20,468 (DSZM), F. nucleatum subsp. nucleatum Knorr (ATCC 25,586), and Escherichia coli Nissle 1917 were used as positive controls for the respective PCR reactions.

Techniques: Bacteria, Marker, Quantitative Proteomics

Journal: Scientific Reports

Article Title: Parvimonas micra as a putative non-invasive faecal biomarker for colorectal cancer

doi: 10.1038/s41598-020-72132-1

Figure Lengend Snippet: Performance of single faecal microbial markers or combinations of markers in CRC detection. Sensitivity and specificity for CRC detection is displayed for a test of ( A ) P. micra ( Pm ), F. nucleatum ( Fn ), or clbA + bacteria ( clbA ), as well as combined tests using several microbial markers for the FECSU and U-CAN cohort, and ( B ) for combined tests using microbial markers and immunochemical F-Hb (Hb) for the FECSU cohort. For test 1, a positive test result was given to samples with at least one positive marker. For test 2, a positive test result was given to samples with at least two positive markers.

Article Snippet: P. micra 20,468 (DSZM), F. nucleatum subsp. nucleatum Knorr (ATCC 25,586), and Escherichia coli Nissle 1917 were used as positive controls for the respective PCR reactions.

Techniques: Bacteria, Marker

Journal: Scientific Reports

Article Title: Parvimonas micra as a putative non-invasive faecal biomarker for colorectal cancer

doi: 10.1038/s41598-020-72132-1

Figure Lengend Snippet: The distribution of specific microbial markers in faeces of CRC patients. Circle diagrams are used to illustrate the abundance of P. micra ( Pm ), F. nucleatum ( Fn ) and clbA + bacteria ( clbA ) in faecal samples with all three markers evaluated of control patients, and patients diagnosed with dysplasia or CRC from the FECSU and U-CAN cohorts.

Article Snippet: P. micra 20,468 (DSZM), F. nucleatum subsp. nucleatum Knorr (ATCC 25,586), and Escherichia coli Nissle 1917 were used as positive controls for the respective PCR reactions.

Techniques: Bacteria, Control

Journal: Scientific Reports

Article Title: Parvimonas micra as a putative non-invasive faecal biomarker for colorectal cancer

doi: 10.1038/s41598-020-72132-1

Figure Lengend Snippet: Microbial alterations in faeces of study patients from the FECSU cohort.

Article Snippet: P. micra 20,468 (DSZM), F. nucleatum subsp. nucleatum Knorr (ATCC 25,586), and Escherichia coli Nissle 1917 were used as positive controls for the respective PCR reactions.

Techniques: Control, Bacteria